Cell Confluency: When It Works—and When It Fails
Cell confluency is easy to measure and easy to misuse. This piece explains what it truly represents, how it is measured, and why it should be interpreted with caution.
Doubling Time, Passage Number & PDL: Everything You Need to Know!
A quick guide to passage number, doubling time, and PDL, what they mean, how to calculate them, and why they matter for reproducible cell culture.
AI Cell Segmentation: Why Universal Models Fall Short in Real Microscopy
Summary General-purpose AI models like Segment Anything (SAM) and CellPose have transformed computer vision and brought powerful new capabilities into the life sciences. But real microscopy data is messy: imaging conditions and quality vary, staining intensity differs, debris and dust appear, and various assays demand very distinct readouts. This article reviews why AI cell segmentation […]
Everything you need to know about Cell Culture
Summary Whether you’re diving into your first biology project or setting up a new lab, cell culture is a foundational technique in the life sciences. This comprehensive guide introduces the essentials of cell culture, from its origins and purpose to protocols, troubleshooting, and expert tips. Bonus: A one-page printable PDF protocol is available at the […]
The Biggest AI Challenge in Biomedical Research
Introduction Artificial intelligence (AI) has transformed many fields, from image recognition to drug discovery, yet its integration into biomedical research remains slower than anticipated. While discussions often center around computational power, algorithms, and cloud infrastructure, the real challenge is far more fundamental: data quality and annotation. A recent poll we conducted reinforced this reality—71% of […]
Transwell Migration and Invasion Assay – the complete breakdown
You have heard about the Transwell Migration Assay and the Invasion Assay, but what exactly are they, and importantly, what is the difference? How are they used, what do they tell you about your cells in culture, and how are they best quantified? This blog post will give you the complete breakdown of the assays and answer all of your questions.
Did You Know? The Impact of Confluency Analysis
This blog contains key papers on the impact of cell confluency analysis. Understand how different parameters in cell culture and cell confluency can affect your experiments, and how to use cellular imaging to your advantage. The dynamic nature of cell culture means that everyday, more discoveries are being made about how confluency and different culture […]
Cellular artistry: The Beauty of Visualizing Cell Confluency
Summary Art and science, although seemingly very different, often coexist and complement each other. In some cases, one can even help you understand the other. This is especially true for cellular biology. For those of us who work in the field, we can attest to how cellular biology can be both informative and beautiful. As […]
Recent Papers using AI and ML that We Like
Using AI and ML in Assay Development or Improvement – Image Analysis, our Favourite! We like these papers because image analysis is just so impactful in assay development. Here are two exciting takes on new assays: 3D cell culture viability and lipid droplet analysis in biofuel production. Nondestructive, quantitative viability analysis of 3D tissue cultures […]
Everything You Need to Know About Cell Confluency
Cell confluency is crucial to consideration in cell culture. These 6 parameters are most important for cell growth; each method has advantages & disadvantages
