Measure Cell Proliferation Using Confluency

Image-Based Cell Proliferation Assay

Cell proliferation plays a central role in cancer biology, drug screening, and regenerative research. However, traditional cell proliferation assay methods—like MTT, WST-1, or PrestoBlue—require destructive sampling and introduce variability across time points.

SnapCyte™ offers a smarter solution. By using cell confluency as a non-invasive proxy for proliferation, our image-based assay delivers accurate, reproducible results—without the need for dyes, extra reagents, or sacrificing any cells.

🔗 Want to learn more? Read our deep dive on cell confluency here.

AI measuring cell confluency to track proliferation
Label-free cell confluency analysis infographic from SnapCyte

How it works

How SnapCyte™ Proliferation Assay Work

SnapCyte™ tracks cell proliferation by measuring confluency across time.

First, culture and treat your cells as usual. Then, capture brightfield or phase-contrast images using either a digital microscope or your smartphone with the SnapCyte™ adapter. Upload your images to the SnapCyte™ app, and our AI analyzes cell density changes over time.

Because SnapCyte™ uses image-based measurements, it preserves your samples and generates a full proliferation curve from confluency data—all without disrupting the experiment.

📹 Scroll down to watch a short video on capturing images with your smartphone.

Why Use SnapCyte™ for Cell Proliferation?

  • Non-Invasive Monitoring
    Track cell confluency over time to infer proliferation without dyes or cell death.

  • Accurate and Reproducible
    Our AI model applies standardized criteria across all time points and users.

  • Save Time and Reagents
    No need for reagents, destructive sampling, or multiple replicates per time point.

  • Integrated Data & Images
    SnapCyte™ keeps images, confluency plots, and growth curves together—ready for export or review.

Get Your Free Academic License

SnapCyte™ is free for academic use. Run a full cell proliferation assay using just images—no staining, extra reagents, or complex setups needed.

Frequently asked questions

Most Frequent Questions and Answers

SnapCyte™ is compatible with a wide range of adherent cell types making it versatile for various research applications. You can also send sample images for analysis before starting your trial to contact@snapcyte.com.

The number of images needed per well depends on the format of the culture plate used and homogeneity of your culture. For example, in a 96-well plate, capturing 2 images at 10X magnification per well is typically sufficient for accurate data.

SnapCyte™ offers an adapter that allows you to acquire images using your smartphone and any basic microscope. It can also work with phase-contrast images taken with other microscopes, providing flexibility in how you capture and upload your data.

Yes, SnapCyte™ allows for continuous monitoring of cell proliferation by analyzing images captured at multiple time points, providing reliable data throughout your experiments.

Analyzing each photo with SnapCyte™ takes less than 30 seconds. For example, if you’re doing an experiment in a 24-well plate, you need to take at least 3 images per well. So, it typically takes about 15 minutes to capture the whole plate.

Yes, SnapCyte™ offers comprehensive customer support, including detailed protocols and user guides in our help centre.